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. Author manuscript; available in PMC: 2013 Dec 4.

Published in final edited form as: Chem Res Toxicol. 2012 Jan 9;25(3):10.1021/tx200471x. doi: 10.1021/tx200471x

Table 1.

Steady-state Kinetic Parameters for Conjugation of Three DEB Stereoisomers with GSH by GSTs

	S,S-DEB			R,R-DEB			meso-DEB
GST	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ min⁻¹)	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ min⁻¹)	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹min⁻¹)
5-5	3.83 ± 0.50	0.77 ± 0.02	5.0 ± 0.8	3.44 ± 0.34	0.80 ± 0.06	4.3 ± 0.1	1.85 ± 0.03	0.83 ± 0.15	2.3 ± 0.4
T1-1	3.71 ± 0.13	0.79 ± 0.03	4.7 ± 0.4	3.70 ± 0.17	0.93 ± 0.01	4.0 ± 0.2	2.42 ± 0.12	0.37 ± 0.01	6.5 ± 0.3
A1-1	1.37 ± 0.02	0.30 ± 0.05	4.7 ± 0.7	1.31 ± 0.22	0.32 ± 0.01	4.1 ± 0.1	2.05 ± 0.04	0.54 ± 0.03	3.8 ± 0.1
A3-3	1.52 ± 0.16	0.48 ± 0.33	4.0 ± 2.4	1.68 ± 0.05	0.51 ± 0.08	3.3 ± 0.4	1.81 ± 0.19	0.75 ± 0.12	2.4 ± 0.1
M1-1	50.4 ± 0.96	0.73 ± 0.06	7.0 ± 1.9	4.44 ± 0.39	0.81 ± 0.05	5.5 ± 0.1	2.98 ± 0.35	0.92 ± 0.33	3.4 ± 0.8
P1-1	2.64 ± 0.18	0.74 ± 0.33	3.9 ± 1.5	2.65 ± 0.01	0.91 ± 0.01	2.9 ± 0.1	1.59 ± 0.03	0.59 ± 0.15	2.8 ± 0.7