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. 2013 Dec 4;8(12):e76963. doi: 10.1371/journal.pone.0076963

Figure 3. Microgravity increases OPC numbers and oligodendrocytes remained immature.

Figure 3

Mixed glial cultures of PLP-GFP labeled OPCs were treated during 24, 48 and 72 h in 0G. (A) Yellow arrowheads define PLP-GFP/Sox2 double positive cells at 24, 48 and 72 h. Green: PLP-GFP, Red: Sox2 immunostaining. Scale bar = 50 µm. (B and C) After treatment, cells were fixed and immunostained for several oligodendrocyte stage markers and the percentage of positive cells in each experimental condition was analyzed by confocal microscopy. (D) Mixed glial cultures of PLP-GFP labeled OPCs were treated during 10, 15 and 30 days in 0G. After treatment, cells were fixed and immunostained for Olig1 and Olig2 and the percentage of positive cells in each experimental condition was analyzed by confocal microscopy. Results are the means ± SEM for three independent experiments. *p<0,05, **p<0,01 and ***p<0,001 vs. respective controls.