Figure 5.

Model building of the 3D structure of the soybean WRKY protein (Glyma13g00380) based on similarity to theAtWRKY4-C domain (Protein Data Bank (PDB) code: 2lexA). The ensemble of the selected structures in stereo view (A), (B), (C), and (D) positive selection sites detected by the branch-site model presented in group I, group II c, group II e, and group III, respectively. The sites with red color indicate amino acid residues under statistically significant (p < 0.05) positive selection, as calculated by Bayes Empirical Bayes estimation methods. The presented region is Asp247–Pro306, excluding the N-terminal region. The figure was produced using the Swiss-model and pyMOL programs.