Figure 2.
Intracellular calcium-releasing channel binding assay. Binding activity of IP3R and RyR was measured as described in Methods. Panels A and C show daily patterns of the binding activity calculated for the IP3R in the ER and PM fractions, respectively. Panels B and D compare the feeding condition groups (Fasted and Refed) for IP3R binding in the ER and PM fractions, respectively. Panel E shows the daily pattern of the RyR binding activity. Panel F compares feeding condition groups for RyR-binding activity. Black circles correspond to AL, and white circles to RF group. The light gray rectangle above x-axis indicates meal time for the food restricted group (ZT4-ZT6). Mean values for four separate experiments are shown. Each data point was carried out in triplicate. * (p < 0.05) significant difference between AL time points and + (p < 0.05) significant difference in the RF group between their time points (1-way ANOVA); # (p < 0.05) significant between AL vs RF (2-way ANOVA). ♦ (p < 0.05) significant difference between Fasted and Refed groups; x (p < 0.05) significant difference between Fasted and RF-ZT3; and & (p < 0.05) significant difference between Refed and RF-ZT6 (Student´s t-test).