Figure 5.

Western blots of hepatic SERCA and PMCA. The daily rhythm profiles and representative western blots of the Ca²⁺-ATPases SERCA2, PMCA1, and PMCA4 are shown in panels A, C, and E, respectively. Comparisons among the different feeding conditions are shown in panels B, D, and F for expression of SERCA2, PMCA1, and PMCA4, respectively. Mean values for at less 4 independent experiments are shown. For SERCA2 protein, 100 μg of ER fraction was used. The plasma membrane fraction (100 μg) was used in the case of PMCA type 1 and 4 proteins. Black circles correspond to AL, and white circles to RF group. The light gray rectangle above x-axis indicates mealtime for the food restricted group (ZT4-ZT6). Mean values for at least 4 independent experiments are shown. Each data point was measured in triplicate. + (p < 0.05) significant difference in the RF group between their time points (1-way ANOVA); # (p < 0.05) significant between AL vs RF (2-way ANOVA). x (p < 0.05) significant difference between Fasted and RF-ZT3 (Student´s t-test).