Table 1.

Primers Used for KIF5A Amplification and DNA Sequencing

	Primer		Annealing Temperaturea(°C)
Exon(s)	Forward	Reverse	Buffer 1	Buffer 2
1	tctgtccccagagactgagcacctg	gaagaggatgaaggatgagcgg		60
2–6	ggccattctcccacatttc	ccctaggcacatctcattcc		55
4b	aagcaatggaagtccaaagg
5b	cttgactccctttccggtta
7	gctgagattcttgacactgcac	ccacagacaaggggtcctc	55
8	gaggaggaccccttgtctgt	atcgtgccactgcaaacc	55
9	ccagccaagcatctctgtta	tcaacagggaatcagaaaggat	55
10	ccatatgatcatgccccaat	ctgggtgaggagaagaggaa	55
11	aagtccaccctgatgtttgg	cagaaacacagcccctcttc	55
12	cctcccatactcccaaaagg	tccacatttcaagcataagca	55
13	gcttcaaccgatcttcctgt	ggagtgattaatccccaagc	55
14	aggcaatgtgcccaccta	tcacaatgcactgaacagca	55
15	ctgcactttcccctcacagt	gcgcccaacctagaatgtat	55
16	ggcggaggaatttaatgtga	cccagcttttctgtccttga	55
17	aaaggaagagccccatagga	gagctacatgcaaaaccttgg	55
18 and 19	cccttgttctctgttcctgct	gcattgttgagggaatgaca	55
20	aatgccacaacttcgtagcc	cgtccctaccccatcatattt	55
21	gtctgtggtcccagatgctt	gccagataccaggtctgtcc	55
22 and 23	ttttgcagccattctgtagc	agggaaagtaaaagcgaacag	55
24 and 25	ggccttttgaggaaatgaca	gcatgaagttgagaaagggttt	55
26	gtggccatgtttgttttcct	ggctgagtgcagtggctatt	55
27	taagatgggagagggtttgc	gggcacaagggaaatttaatc	55
28	aatagtccctgccgtttgtg	ggaagcagcctgacactacc	55

^aBuffer 1: 10× buffer (100 mM Tris, 500 mM KCl, 10 mM MgCl₂, 1% Triton X-100, and 0.1% gelatin). Buffer 2: 10× buffer (100 mM Tris, 15 mM MgCl₂, and 500 mM KCl).

^bNested sequencing primers for exons 4 and 5.