. 2013 Oct 5;14:45. doi: 10.1186/1471-2121-14-45

Table 2.

ADF and cofilin suppression affects MTLn3 cell polarization after EGF stimulation

EGF stimulation		Subcategory	Control	ADF KD	Cofilin KD
0 sec	Polarized	Crescent-shaped	68.3 ± 1.6	33.6 ± 1.2^***	13.1 ± 0.6^***
	Polarized	Kite-shaped	31.7 ± 1.6	66.4 ± 1.2^***	86.4 ± 0.6^***
	Non-polarized	Apolar	91.3 ± 0.3	91.0 ± 0.1	71.5 ± 1.3
		Bipolar	8.7 ± 0.3	9.0 ± 0.1	9.7 ± 1.2
		Multipolar	0.0	0.0	18.8 ± 1.5
60 sec	Polarized	Crescent-shaped	75.0 ± 1.1	75.2 ± 1.9	49.3 ± 0.7^***
	Polarized	Kite-shaped	25.0 ± 1.1	24.8 ± 1.9	50.7 ± 0.7^***
	Non-polarized	Apolar	91.4 ± 0.3	66.6 ± 0.2^***	100.0^***
		Bipolar	8.6 ± 0.3	34.4 ± 0.2^***	0.0
		Multipolar	0.0	0.0	0.0
180 sec	Polarized	Crescent-shaped	79.8 ± 0.2	80.6 ± 0.4	48.5 ± 0.5^***
	Polarized	Kite-shaped	20.2 ± 0.2	19.4 ± 0.4	51.5 ± 0.5^***
	Non-polarized	Apolar	91.2 ± 0.3	95.7 ± 0.3^***	100.0^***
		Bipolar	8.8 ± 0.3	4.3 ± 0.3^***	0.0
		Multipolar	0.0	0.0	0.0

Polarized and non-polarized cells were subcategorized as in Figure 4A and the percentage of cells in each category was scored. Data are expressed as mean ± SEM, n ≥ 250 cells in each experiment, three independent experiments. ***p < 0.001 versus control.