Table 2.
EGF stimulation | Subcategory | Control | ADF KD | Cofilin KD | |
---|---|---|---|---|---|
0 sec |
Polarized |
Crescent-shaped |
68.3 ± 1.6 |
33.6 ± 1.2*** |
13.1 ± 0.6*** |
Kite-shaped |
31.7 ± 1.6 |
66.4 ± 1.2*** |
86.4 ± 0.6*** |
||
Non-polarized |
Apolar |
91.3 ± 0.3 |
91.0 ± 0.1 |
71.5 ± 1.3 |
|
Bipolar |
8.7 ± 0.3 |
9.0 ± 0.1 |
9.7 ± 1.2 |
||
Multipolar |
0.0 |
0.0 |
18.8 ± 1.5 |
||
60 sec |
Polarized |
Crescent-shaped |
75.0 ± 1.1 |
75.2 ± 1.9 |
49.3 ± 0.7*** |
Kite-shaped |
25.0 ± 1.1 |
24.8 ± 1.9 |
50.7 ± 0.7*** |
||
Non-polarized |
Apolar |
91.4 ± 0.3 |
66.6 ± 0.2*** |
100.0*** |
|
Bipolar |
8.6 ± 0.3 |
34.4 ± 0.2*** |
0.0 |
||
Multipolar |
0.0 |
0.0 |
0.0 |
||
180 sec | Polarized |
Crescent-shaped |
79.8 ± 0.2 |
80.6 ± 0.4 |
48.5 ± 0.5*** |
Kite-shaped |
20.2 ± 0.2 |
19.4 ± 0.4 |
51.5 ± 0.5*** |
||
Non-polarized | Apolar |
91.2 ± 0.3 |
95.7 ± 0.3*** |
100.0*** |
|
Bipolar |
8.8 ± 0.3 |
4.3 ± 0.3*** |
0.0 |
||
Multipolar | 0.0 | 0.0 | 0.0 |
Polarized and non-polarized cells were subcategorized as in Figure 4A and the percentage of cells in each category was scored. Data are expressed as mean ± SEM, n ≥ 250 cells in each experiment, three independent experiments. ***p < 0.001 versus control.