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Model structure representation of the wild and mutant (Glu155Lys) proteins. A: Glu155 forms two hydrogen bonds, one with side chain amide nitrogen and another with main chain nitrogen of Asn162 which stabilizes and maintains the loop conformation essential for interactions with other proteins. B: In mutant (Glu155Lys) proteins, Asn162 flips by approximately 180° and forms hydrogen bond with the side chain nitrogen atom of the mutated residue Lys155.
