Table 1. Oligonucleotides of mutated genes used as primers for PCR amplification.

Gene Name	Primer Name	Primer Sequence	Anne Temp (°C)	Product Size (bp)
CRYBA4	Ex-2-F	5′-TAGCCCAGTCACTCCTGGAC-3′	57 °C	213
	Ex-2-R	5′-GCCTTGATTGCACCTCTGTG-3′
	Ex-3-F	5′-TTTGCAATCCCTGCTTTACC-3′	57 °C	423
	Ex-3-R	5′-ATGGCACCCTCCTACTGTTGG-3′
	Ex-4-F	5′-AAAAATGTCTCCAGCCATCG-3′	57 °C	314
	Ex-4-R	5′-AGCTTGAAGTGGCGACATGAG-3′
	Ex-5-F	5′-AAATGGCAAGGTTTCTGGTAC-3′	57 °C	297
	Ex-5-R	5′-GCCTCAGTGTTCTCCTCTGG-3′
	Ex-6-F	5′-AGGGAATGGCATGATCAAAG-3′	57 °C	335
	Ex-6-R	5′-TGCTGGGTTCACACAGGTTAC-3′
CRYBB1	Ex-2-F	5′-ACAGGATGTGGGGCTATGAG-3′	59 °C	380
	Ex-2-R	5′-GTGCGGAGGAGTAAGAGGTG-3′
	Ex-3-F	5′-CATTTCACAAACTGTGGCTCA-3′	62 °C	379
	Ex-3-R	5′-GGACATAATGTATGTGCCAGGA-3′
	Ex-4-F	5′-GTAGGGAGTGGGGGCTTCTA-3′	62 °C	286
	Ex-4-R	5′-CTCCTTCTTGCCCTTGTCAG-3′
	Ex-5-F	5′-GCTCATCTCTCTCGCTCCAC-3′	61 °C	298
	Ex-5-R	5′-TCTGATTCTGCCTGTGCTTG-3′
	Ex-6-F	5′-TCAATGAAGGACAGGCTGGT-3′	62 °C	381
	Ex-6-R	5′-TCCAGGAGAAATTTTGGCTTT-3′
CRYBB2	Ex-2-F	5′-CAGAGGGGAGTGGTCTCAAG-3′	59 °C	244
	Ex-2-R	5′-ATGCCAAGCCCATTTTACAG-3′
	Ex-3-F	5′-TCAGCATCCTTTGGGTTCTC-3′	59 °C	299
	Ex-3-R	5′-CAAGGGTAGATTCCCCCACT-3′
	Ex-4-F	5′-AACCCTAGGGGTCAACATCA-3′	62 °C	297
	Ex-4-R	5′-CTCCAAGGTGGCAGAGAGAG-3′
	Ex-5-F	5′-GAGTGATGTGTGGGACATGC-3′	62 °C	377
	Ex-5-R	5′-CAGAGGTCAGCAGAGCACAC-3′
	Ex-6-F	5′-GGCTTCACCCTTCCTAGTGG-3′	59 °C	399
	Ex-6-R	5′-CAAAGACCCACAGCAGACAA-3′
GAJ3	Ex-1A-E	5′-TGCGGACCCGGCACTCAGC-3′	62 °C	383
	Ex-1A-R	5′-TCCATGCGCACGATGTGCAGTCA-3′
	Ex-1B-F	5′-CTGTTCATCTTCCGCATTTTGG-3′	62 °C	603
	Ex-1B-R	5′-TCTTCTTCCAGCCCAGGTGGTA-3′
	Ex-1C-F	5′-AAGCTCAAGCAGGGCGTGAC-3′	62 °C	624
	Ex-1C-R	5′-CTAGATGGCCAAGTCCTCCGG-3′
BFSP1	Ex-1-F	5′-GGGCCTCCGGTGTTTATTTA-3′	58 °C	589
	Ex-1-R	5′-ATCGACAGGGGACCGAGAGAC-3′
	Ex-2-F	5′-AAAGGAGAGGGCATCGTACC-3′	58 °C	238
	Ex-2-R	5′-AACCTGCACTTCCACCATTC-3′
	Ex-3-F	5′-CAGGTGGTCTGTGTGCACAT-3′	58 °C	249
	Ex-3-R	5′-TCGGCTTACCTGATCAAACC-3′
	Ex-4-F	5′-RGCCATTCCTGTTCTCATCT-3′	58 °C	250
	Ex-4-R	5′-GCCCTTCCCTGGGAGTCT-3′
	Ex-5-F	5′-ACCTTCTCTGCCCTTTTCCT-3′	58 °C	227
	Ex-5-R	5′-CACCTCCATGAAACATGTGG-3′
	Ex-6-F	5′-CCTTTTCCTGGGTGAGGTCTG-3′	58 °C	366
	Ex-6-R	5′-GGCACACAATAGGCACTCAA-3′
	Ex-7-F	5′-CTTGCCCCTGACCTCTGTT-3′	58 °C	199
	Ex-7-R	5′-AAGAGAGCCGCTTGGTTTTT-3′
	Ex-8-F	5′-TTCCAACCAGCGTATTTTCTTT-3′	58 °C	699
	Ex-8-R	5′-TCAGGGCCTTCCAGCTCT-3′
	In5-Ex-7-F	5′-CATCTTCCAGGGTGTCCAG-3′	58 °C	316
	In5-Ex-7-R	5′-AAGAGAGCCGCTTGGTTTTT-3′