Table 1.
Comparison of m/z values of N-terminal AspN-digested/tryptic peptides of LprF, LpqH, LpqL and LppX found in BCG parental and Δlnt mutant strain
| Peptide | Calculated m/z | Parental strain m/z | Δlnt m/z | |
|---|---|---|---|---|
|
LprF |
CGK…ILQ |
2496.24 |
- |
- |
|
CGK…ILQ |
3047.11 |
- |
3046.70 |
|
| + Diacylglycerol (C16/C16) |
(+550.87) |
|
(+550.46) |
|
|
CGK…ILQ |
3073.15 |
- |
3072.71 |
|
| + Diacylglycerol (C16/C18) |
(+576.91) |
|
(+576.47) |
|
|
CGK…ILQ |
3089.20 |
- |
3088.74 |
|
| + Diacylglycerol (C16/C19) |
(+592.96) |
|
(+592.50) |
|
|
CGK…ILQ |
3251.44 |
- |
3251.65 |
|
| + Diacylglycerol (C16/C19) |
(+755.20) |
|
(+755.41) |
|
| + Hexose |
|
|
|
|
|
CGK…ILQ |
3327.60 |
3326.83 |
- |
|
| + Diacylglycerol (C16/C19) |
(+831.36) |
(+830.59) |
|
|
| + N-acyl (C16) |
|
|
|
|
|
CGK…ILQ |
3531.93 |
3530.56 |
- |
|
| + Diacylglycerol (C16/C19) |
(+1035.69) |
(+1034.32) |
|
|
| + N-acyl (C19) |
|
|
|
|
| |
+ Hexose |
|
|
|
|
LpqH |
CSSNK |
538.23 |
- |
- |
|
CSSNK |
1089.10 |
- |
1088.60 |
|
| + Diacylglycerol (C16/C16) |
(+550.87) |
|
(+550.37) |
|
|
CSSNK |
1115.14 |
- |
1114.68 |
|
| + Diacylglycerol (C16/C18) |
(+576.91) |
|
(+576.45) |
|
|
CSSNK |
1131.19 |
1130.79 |
1130.71 |
|
| + Diacylglycerol (C16/C19) |
(+592.96) |
(+592.56) |
(+592.48) |
|
|
CSSNK |
1369.59 |
1369.04 |
- |
|
| + Diacylglycerol (C16/C19) |
(+831.36) |
(+830.81) |
|
|
| |
+ N-acyl (C16) |
|
|
|
|
LpqL |
CIR |
391.21 |
- |
- |
|
CIR |
984.17 |
984.50 |
983.77 |
|
| + Diacylglycerol (C16/C19) |
(+592.96) |
(+593.29) |
(+592.56) |
|
|
CIR |
1222.57 |
1221.98 |
- |
|
| + Diacylglycerol (C16/C19) |
(+831.36) |
(+830.77) |
|
|
| |
+N-acyl (C16) |
|
|
|
|
LppX |
CSS…EIR |
2964.46 |
- |
- |
|
CSS…EIR |
3515.33 |
3514.94 |
3514.94 |
|
| + Diacylglycerol (C16/C16) |
(+550.87) |
(+550.48) |
(+550.48) |
|
|
CSS…EIR |
3557.42 |
- |
3556.96 |
|
| + Diacylglycerol (C16/C19) |
(+592.96) |
|
(+592.50) |
|
|
CSS…EIR |
3719.66 |
- |
3719.00 |
|
| + Diacylglycerol (C16/C19) |
(+755.20) |
|
(+754.54) |
|
| +Hexose |
|
|
|
|
|
CSS…EIR |
3795.82 |
3795.21 |
- |
|
| + Diacylglycerol (C16/C19) |
(+831.36) |
(+830.75) |
|
|
| + N-acyl (C16) |
|
|
|
|
|
CSS…EIR |
3881.90 |
- |
3881.06 |
|
| + Diacylglycerol (C16/C19) |
(+917.44) |
|
(+916.60) |
|
| + 2 Hexoses |
|
|
|
|
|
CSS…EIR |
3958.06 |
3957.28 |
- |
|
| + Diacylglycerol (C16/C19) |
(+993.60) |
(+992.82) |
|
|
| + N-acyl (C16) |
|
|
|
|
| + Hexose |
|
|
|
|
|
CSS…EIR |
4120.30 |
4119.45 |
- |
|
| + Diacylglycerol (C16/C19) |
(+1155.84) |
(+1154.99) |
|
|
| + N-acyl (C16) |
|
|
|
|
| + 2 Hexoses |
Peptides correspond to the N-terminal AspN-digested/tryptic peptides of LprF, LpqH, LpqL and LppX upon cleavage of the signal peptide by LspA. Mass differences to the corresponding unmodified peptide (bold number) due to modifications are given in parentheses. Observed modifications are: diacylglycerol with C16 fatty acid and C16 fatty acid (+550.87 Da). Diacylglycerol with C16 fatty acid and tuberculostearic acid (C19:0) (+592.96 Da), plus one hexose (+162.24 Da, Σ = 755.20 Da) or two hexoses (+324.48 Da, Σ = 917.44). Diacylglycerol with C16 fatty acid and C19:0 fatty acid (+592.96 Da) plus N-acyl with C16 fatty acid (+238.40 Da, Σ = 831.36), N-acyl with C16 fatty acid plus one hexose (+162.24 Da, Σ = 993.6 Da) or two hexoses (+324.48 Da, Σ = 1155.84 Da). Or diacylglycerol with C16 fatty acid and C19:0 fatty acid (+592.96 Da) plus N-acyl with C19:0 fatty acid and hexose (+280.49 Da +162.24 Σ = 1035.69).