Table 2.
Comparison of experimentally determined eliminations from N-terminal AspN digested/tryptic peptides of LprF, LpqH, LpqL and LppX in the MALDI-TOF/TOF spectra of BCG parental and Δlnt mutant strain with theoretically calculated eliminations
| Modification | Eliminated fragment | Calculated mass of eliminated fragment [ Da ] |
Experimentally determined mass of eliminated fragment [ Da ] |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Parental strain |
Δlnt |
|||||||||||||
|
LprF |
LpqH |
LpqL |
LppX |
LprF |
LpqH |
LpqL |
LppX |
|||||||
| C16/C19 C16 | C16/C19 C19 | C16/C19 C16 | C16/C19 C16 | C16/C16 | C16/C19 | C16/C16 | C16/C18 | C16/C19 | C16/C19 | |||||
|
O-linked palmitoyl (C16) |
Palmitic acid |
256.24 |
256.5 |
- |
256.3 |
256.3 |
n.d. * |
- |
- |
256.2 |
256.1 |
256.3 |
256.3 |
n.d. * |
|
O-linked oleyl (C18) |
Oleic acid |
282.24 |
- |
- |
- |
- |
n.d. * |
- |
- |
- |
282.4 |
- |
- |
n.d. * |
|
O-linked tuberculostearyl (C19) |
Tuberculostearic acid |
298.29 |
- |
- |
298.3 |
298.3 |
n.d. * |
- |
- |
- |
- |
298.3 |
298.4 |
n.d. * |
|
N-linked palmitoyl (C16) + Didehydroalanine |
Palmitamide + Didehydroalanine |
307.26 |
- |
306.6 |
- |
- |
n.d. * |
- |
- |
- |
- |
- |
- |
n.d. * |
|
N-linked tuberculostearyl (C19) + Didehydroalanine |
Tuberculostearinamide + Didehydroalanine |
349.31 |
349.8 |
- |
- |
- |
n.d. * |
- |
- |
- |
- |
- |
- |
n.d. * |
| Diacylglyceryl (C16/C16) |
Diacylhioglyceryl (C16/C16) |
584.44 |
- |
- |
- |
- |
n.d. * |
583.3 |
- |
- |
- |
- |
- |
n.d. * |
| Diacylglyceryl (C16/C18) |
Diacylhioglyceryl (C16/C18) |
610.52 |
- |
- |
- |
- |
n.d. * |
- |
- |
- |
- |
- |
- |
n.d. * |
| Diacylglyceryl (C16/C19) |
Diacylhioglyceryl (C16/C19) |
626.53 |
625.9 |
626.7 |
626.7 |
626.6 |
n.d. * |
- |
626.7 |
- |
- |
626.6 |
626.7 |
n.d. * |
| |
C16 fatty acid α-thioglyceryl ester |
328.24 |
- |
- |
328.4 |
328.3 |
n.d. * |
- |
|
- |
- |
- |
- |
n.d. * |
| |
C19 fatty acid α-thioglyceryl ester |
370.29 |
- |
- |
370.5 |
370.3 |
n.d. * |
- |
369.8 |
- |
- |
- |
370.4 |
n.d. * |
| Hexose | Hexose | 160.76 | 161.62 | - | - | - | n.d. * | - | 162.9 | - | - | - | - | n.d. * |
* MALDI-TOF/TOF data for LppX from M. bovis BCG were not determined, since MS data of LppX from this study are comparable with data of LppX from M. smegmatis (A. Tschumi et al. 2009).