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. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: Circ Res. 2013 Jul 12;113(5):10.1161/CIRCRESAHA.113.301792. doi: 10.1161/CIRCRESAHA.113.301792

Figure 2. Multipotent marker expression in Mgp−/− and Mgp−/−;Tie2-Gfptg endothelium.

Figure 2

(A) Aortic expression of Sox2, Nanog and Oct3/4 in Mgp+/+ and Mgp−/− mice detected by immunoblotting (left) and immunostaining (right). β-actin was used as control. (B) Aortic expression of Sox2, Nanog and Oct3/4 in Tie2-Gfptg and Mgp−/−;Tie2-Gfptgmice determined by immunoblotting (left) and immunostaining (right). β-actin was used as control. (C) Co-expression of CD31 and Sox2 in enzymatically dispersed CD45-negative aortic cells from Mgp+/+ and Mgp−/− mice, as determined by FACS. Scale bars, 50 μm. Non-specific IgG control showed no staining. Vessel lumen faces upwards in the photos.