Table 4.
Primer sequences used in qPCR for SCNN1 gene family members and internal control gene β-actin
| Primer name | Sequence (5’→3’) | Tm(°C) | Product size (bp) | Position |
|---|---|---|---|---|
| SCNN1a-F |
TCATGTTCAGCGCCATCCT |
60 |
175 |
Exon3+4 628-802 |
| SCNN1a-R |
TTCCCGCACTGCATCCA |
60 |
|
|
| SCNN1b-F |
GAAGTTCCCAGCAGTCACAGTCT |
60 |
168 |
Exon2+3 277-444 |
| SCNN1b-R |
GTGGCGTTGCTGTTGTTCAG |
60 |
|
|
| SCNN1d-F |
CACCATCCACGGCACCAT |
60 |
183 |
Exon1 90-272 |
| SCNN1d-R |
AACATCTTGGGCTCCGAATG |
60 |
|
|
| SCNN1g-F |
TGGGACAAAGGACAGAAAATC |
60 |
141 |
Exon10+12 1450-1590 |
| SCNN1g-R |
GCCGAAGTTGGACAGAAGGA |
60 |
|
|
| β-actin-F |
TATGTGCAAGGCCGGTTTC |
60 |
110 |
Exon1+2 113-222 |
| β-actin-R | TGTCTTTCTGGCCCATACCAA | 60 |