Table 1. Effect of metal chelators, detergents, electron donors on the LmbB2 activity.
Additive | Concentration | Relative activity (%) |
None | - | 100 |
Chelator | (mM) | |
EDTA | 0.1 | 83 |
1.0 | 73 | |
Detergent | (% w/v) | |
SDS | 0.01 | 85 |
0.02 | 71 | |
Electron donors | (mM) | |
NADH* | 2 | 75 |
NADPH | 2 | 56 |
BH4 * | 2 | 186 |
tetrahydrofolic acid | 2 | 118 |
compounds were tested in concentrations ranging from 2 µm to 2 mM. The results were consistent with the conclusion that BH4 and NADH increase and decrease, respectively, the LmbB2 activity.