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. 2013 Dec 4;8(12):e79861. doi: 10.1371/journal.pone.0079861

Figure 3. In vitro ecdysteroid secretion by ovaries of dsRNA injected females.

Figure 3

(A) RT-PCR analysis of cyp314aA1 in ovaries of dsgfp and dscyp314a1 females 22h after blood-feeding. (B) RT-PCR analysis of cyp307a1 in ovaries of dsgfp and dscyp307aA1 females 22h after blood-feeding. (C) In vitro ecdysteroid secretion of ovaries from dsgfp and dscyp314a1 females 22 h after blood-feeding. Results are expressed as mean ± SEM in 20E equivalents (in pg) per ovaries pair. (D) In vitro ecdysteroid secretion of ovaries from dsgfp and dscyp307a1 females 22 h after blood-feeding. Results are expressed as mean ± SEM in E equivalents (in pg) per ovaries pair. Results were subjected to statistical analysis using Mann-Whitney test (**, P<0.01).