Table 1.
Ceramides after elastase injection
| Ceramide | Day 0 | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 8 | Day 14 | Day 21 |
|---|---|---|---|---|---|---|---|---|---|
| Ceramide 16:0 |
45.63 ± 33.52 |
32.04 ± 14.16 |
110.54 ± 57.84 * |
48.52 ± 3.87 |
21.91 ± 6.73 |
52.84 ± 1.86 |
40.97 ± 21.74 |
46.66 ± 27.52 |
38.04 ± 20.54 |
| Ceramide 18:0 |
2.47 ± 0.68 |
3.55 ± 1.71 |
5.50 ± 1.55* |
2.97 ± 0.48 |
1.38 ± 0.23 |
1.86 ± 0.61 |
2.20 ± 1.18 |
2.11 ± 0.72 |
2.46 ± 1.58 |
| Ceramide 20:0 |
1.39 ± 0.40 |
1.92 ± 0.77 |
4.25 ± 1.98* |
1.86 ± 0.36 |
0.79 ± 0.16 |
1.14 ± 0.40 |
1.41 ± 0.79 |
1.30 ± 0.41 |
1.32 ± 0.83 |
| Ceramide 22:0 |
9.60 ± 2.47 |
14.12 ± 7.82 |
29.75 ± 11.28* |
16.31 ± 2.80 |
6.66 ± 2.30 |
9.00 ± 2.85 |
10.89 ± 5.74 |
10.13 ± 2.12 |
10.40 ± 5.40 |
| Ceramide 24:0 |
34.83 ± 12.23 |
34.97 ± 20.00 |
112.76 ± 47.82* |
44.75 ± 6.09 |
19.09 ± 6.43 |
36.70 ± 16.82 |
40.97 ± 20.87 |
37.58 ± 9.04 |
41.64 ± 19.19 |
| Ceramide 24:1 |
24.89 ± 8.47 |
52.02 ± 37.77 |
95.90 ± 41.08* |
60.25 ± 9.65* |
23.04 ± 9.75 |
33.03 ± 12.09 |
32.90 ± 20.18 |
30.01 ± 8.14 |
31.41 ± 16.8 |
| Dihydro Ceramide 18:0 |
0.53 ± 0.24 |
2.01 ± 0.40* |
1.49 ± 0.53 |
1.51 ± 0.44 |
0.75 ± 0.34 |
0.36 ± 0.10 |
0.48 ± 0.31 |
0.40 ± 0.16 |
0.33 ± 0.15 |
| Dihydro Ceramide 24:0 | 2.17 ± 0.68 | 4.64 ± 1.14 | 10.74 ± 4.32* | 3.77 ± 1.95 | 1.72 ± 0.34 | 1.83 ± 0.85 | 2.21 ± 0.99 | 1.85 ± 0.49 | 1.83 ± 0.85 |
BAL fluid from 12 mice per group was collected and processed using LC-MS/MS. Data are expressed as Mean + SEM ng/ml BAL fluid. *p<0.05, when compared to control animals at the same time-point.