Table 2. FTIR analysis of the WT scFV-h3D6 and its variants at different temperatures.
| |
WT 25°C |
WT 50°C |
WT 60°C |
WT Renat |
||||
|---|---|---|---|---|---|---|---|---|
| Center (cm−1) | Area (%) |
Center (cm−1) | Area (%) |
Center (cm−1) | Area (%) |
Center (cm−1) | Area (%) |
|
| High-freq. antipar. β-sheet |
1688 |
2 |
1689 |
2 |
1685 |
3 |
1685 |
2 |
| β-turns |
1674 |
9 |
1677 |
8 |
1676 |
3 |
1672 |
5 |
|
Loops/turns |
1659 |
22 |
1662 |
22 |
1661 |
30 |
1662 |
17 |
| α-helix |
|
|
|
|
1650 |
7 |
1653 |
10 |
| Random coil |
|
|
|
|
1643 |
7 |
1645 |
6 |
| Native β-sheet |
1637 |
64 |
1637 |
65 |
1636 |
16 |
1637 |
19 |
| WL β-sheet |
|
|
|
|
1627 |
23 |
1626 |
26 |
| Amyloid β-sheet |
|
|
|
|
1616 |
10 |
1616 |
14 |
| Side-chains |
1614a |
3 |
1613a |
3 |
1607 |
1 |
1607 |
1 |
| |
|
|
|
|
||||
| |
C1 25°C |
C1 50°C |
C1 60°C |
C1 Renat |
||||
| |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
| High-freq. antipar. β-sheet |
1685 |
3 |
1689 |
1 |
1685 |
0 |
1685 |
2 |
| β-turns |
|
|
1675 |
13 |
1678 |
9 |
1679 |
8 |
| Loops/turns |
1663 |
30 |
1659 |
21 |
1662 |
16 |
1667 |
14 |
| α-helix |
|
|
|
|
1653 |
13 |
1655 |
10 |
| Random coil |
|
|
|
|
1643 |
18 |
1643 |
19 |
| Native β-sheet |
1637 |
66 |
1637 |
63 |
1632 |
23 |
1632 |
20 |
| WL β-sheet |
|
|
|
|
1623 |
15 |
1621 |
18 |
| Amyloid β-sheet |
|
|
|
|
1614 |
5 |
1614 |
9 |
| Side-chains |
1611 |
1 |
1614a |
2 |
1607 |
1 |
1607 |
2 |
| |
|
|
|
|
||||
| |
C2 25°C |
C2 50°C |
C2 60°C |
C2 Renat |
||||
| |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
| High-freq. antipar. β-sheet |
1688 |
2 |
1683 |
4 |
1683 |
3 |
1681 |
5 |
| β-turns |
1678 |
5 |
1675 |
0 |
1671 |
14 |
1669 |
13 |
| Loops/turns |
1662 |
26 |
1663 |
30 |
1662 |
5 |
1659 |
8 |
| α-helix |
|
|
|
|
1652 |
20 |
1652 |
15 |
| Random coil |
|
|
|
|
1643 |
12 |
1643 |
16 |
| Native β-sheet |
1637 |
66 |
1638 |
65 |
1636 |
15 |
1631 |
23 |
| WL β-sheet |
|
|
|
|
1627 |
18 |
1621 |
15 |
| Amyloid β-sheet |
|
|
|
|
1617 |
11 |
1614 |
5 |
| Side-chains |
1612a |
1 |
1613a |
1 |
1607 |
1 |
1607 |
1 |
| |
|
|
|
|
||||
| |
C3 25°C |
C3 50°C |
C3 60°C |
C3 Renat |
||||
| |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
Center (cm−1) |
Area (%) |
| High-freq. antipar. β-sheet |
1689 |
2 |
1689 |
2 |
1685 |
4 |
1689 |
1 |
| β-turns |
1677 |
9 |
1678 |
10 |
1676 |
7 |
1679 |
9 |
| Loops/turns |
1661 |
22 |
1661 |
23 |
1666 |
6 |
1663 |
18 |
| |
|
|
|
|
1657 |
23 |
1657 |
2 |
| α-helix |
|
|
|
|
1650 |
5 |
1648 |
17 |
| Random coil |
|
|
|
|
1640 |
18 |
1640 |
5 |
| Native β-sheet |
1637 |
65 |
1637 |
63 |
1630b |
21 |
1630b |
30 |
| WL β-sheet |
|
|
|
|
1619b |
15 |
1619b |
13 |
| Amyloid β-sheet |
|
|
|
|
|
|
1613b |
3 |
| Side-chains | 1612a | 2 | 1613a | 1 | 1608 | 1 | 1608 | 2 |
a This band, quantitatively negligible, could also correspond to the amyloid component. bThese bands are difficult to be attributed because they are centered at boundaries.