Table 1. Mass Spectral Characterization of Protein Components Detected in Hetero-AB.
Protein | Theoretical Mass (Da)a,b | Measured Mass (Da) | Mass Error (ppm) | Deconvoluted MS Intensity | Relative Intensity |
---|---|---|---|---|---|
AB - 2Lys |
145321 |
145322 |
7 |
1089472 |
72.5% |
AB - Lys |
145449 |
145449 |
0 |
333951 |
22.2% |
AB | 145577 | 145579 | 14 | 80160 | 5.3% |
A, Theoretical mass is for fully deglycosylated protein; b, Both heavy chain A and B have glycine addition on the N-terminus.