Table 1. Nucleotide and amino acid sequence of V_HCDR2 and V_LCDR1 and their mutants; IC₅₀ and EC₅₀ value tested on D54MG cells shown.

																					IC50 (ng/ml)	EC50 (µM)
Position:			71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88
DmAb14-VHCDR2 Parental Amino Acid			E	I	N	P	S	N	G	R	T	N	Y	N	E	K	F	K	S	K	N/A	0.45
DmAb14-VHCDR2 Mutant#
		No.86	E	I	N	P	M	D	S	R	T	N	Y	N	E	K	F	K	S	K	N/A	0.9
		No.126	E	I	N	P	L	P	P	R	T	N	Y	N	E	K	F	K	S	K	N/A	28
Position:			25	26	27	28	29	30	31	32	33	34	35	36	37	38	39
DmAb14-VLCDR1# Parental Amino Acid			R	A	S	E	S	V	E	S	Y	G	N	N	F	M	H				N/A	0.45
DmAb14-VLCDR1Mutant
	No. 23		R	A	R	E	A	V	E	S	Y	G	N	N	F	M	H				N/A	0.8
	No.104		P	W	R	E	S	V	E	S	Y	G	N	N	F	M	H				N/A	4.7
	No.184		L	Y	G	E	S	V	E	S	Y	G	N	N	F	M	H				N/A	0.2

^# When V_HCDR2 had mutations, V_LCDR1 kept parental residues. When V_LCDR1 had mutations, V_HCDR2 kept parental residues. *Hot-spot positions are underlined and boldfaced.