Table 4. Potency and Relative amounts of PTMs in mAb B by LC-MS peptide mapping.
| Storage Temperature | N55D | M250ox | M426ox | Potency |
|---|---|---|---|---|
|
1 mo time point | ||||
| -80°C |
21.6% |
10.1% |
3.7% |
100% 100% 103% 101% |
| 5°C |
21.7% |
10.0% |
3.4% |
|
| 25°C |
23.9% |
10.8% |
3.8% |
|
| 40°C |
36.4% |
11.7% |
4.1% |
|
|
3 mo time point | ||||
| 5°C |
21.1% |
14.7% |
3.1% |
113% 108% 94% |
| 25°C |
28.1% |
16.7% |
2.7% |
|
| 40°C | 55.6% | 18.6% | 3.5% | |
N55D refers to relative amount of aspartic acid at position 55 on the heavy chain. Met248ox and Met250ox refer to methionine oxidation position 248 and 250. Potency by Binding ELISA is expressed as % of reference standard