Figure 5.

Connexin-mimetic peptides affect injury-induced NO synthesis. 1 hour pre-incubation with either ^37,43Gap27 (300 μM) (A) or ⁴⁰Gap27 (300 μM) (B) dramatically attenuated the increase in intracellular NO levels provoked by endothelial lesion. For sake of clarity, the control curve has been depicted in each Panel. All the experiments depicted in this figure have been carried out on the same day on different rings isolated from the same animal. The control (PSS, closed circles) trace is the mean±SE of 9 cells, while the ^37,43Gap27 (open circles in Panel A) and ⁴⁰Gap27 traces (open circles in Panel B) are, respectively, the mean±SE of 5 and 7 cells. C and D, mean±SE of NOP elicited by injury following the designated treatments. Each drug was applied was 20 min before carrying out the lesion. The asterisk indicates a level of significant difference <0.05. The number of ECs analyzed in each condition is indicated above the bars. See the text for drug concentrations