Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Nov 28;4:18–24. doi: 10.1016/j.fob.2013.11.004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig. 3 — Adipogenic differentiation is more prevalent in Kit^W-sh/W-sh SVF than in Kit^+/+ SVF in vitro. (A) A flow cytometric analysis of the SVF isolated from the Kit^+/+ and Kit^W-sh/W-sh mice fed an ND. c-Kit/FcεRIα double-positive cells were observed within the gates. Representative data from three independent experiments are shown. (B) The mRNA level of MCCPA was evaluated in the Kit^+/+ and Kit^W-sh/W-sh SVF using quantitative RT-PCR. The SVF cells obtained at isolation (freshly isolated), confluence (confluent) and day seven of adipogenic induction (differentiated) were examined (n = 4 for each group). The data are presented as the mean ± SEM. **P < 0.01 versus the Kit^W-sh/W-sh mice. (C) The SVF cells derived from the Kit^+/+ and Kit^W-sh/W-sh mice fed an ND were cultured in the presence of adipogenic factors, then fixed and stained with Oil red O. Oil red O-positive differentiated adipocytes were observed under a microscope (×100). (D) The stained lipids were extracted, and the absorbance was measured at 540 nm. n = 5 for each group. The data are presented as the mean ± SEM. *P < 0.05 for the comparisons indicated. (E) The mRNA levels of the adipocyte marker genes were examined in the differentiated SVF obtained from the Kit^+/+ (black bars) and Kit^W-sh/W-sh (gray bars) mice using quantitative RT-PCR (n = 3 for each group). The data are presented as the mean ± SEM. *P < 0.05 versus the Kit^+/+ mice.