Table 4.

Comparison of Selenomonas ruminantium percentage by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing

		Selenomonas ruminantium (% ± SD)
Sample		qPCR	Shotgun	Amplicon
50% roughage	GL	0.063 ± 0.0003	0.003 ± 0.000	0.400 ± 0.003
	DL	0.040 ± 0.0001	0.005 ± 0.000	0.430 ± 0.001
	GS	0.006 ± 0.000	0.004 ± 0.000	0.750 ± 0.002
	DS	0.008 ± 0.000	0.003 ± 0.000	1.000 ± 0.000
75% roughage	GL	0.090 ± 0.000	0.004 ± 0.000	0.800 ± 0.000
	DL	0.005 ± 0.000	0.002 ± 0.000	0.800 ± 0.000
	GS	0.050 ± 0.000	0.003 ± 0.000	2.000 ± 0.000
	DS	0.001 ± 0.000	0.004 ± 0.000	1.000 ± 0.000
100% roughage	GL	0.002 ± 0.001	0.004 ± 0.000	0.770 ± 0.001
	DL	0.002 ± 0.001	0.002 ± 0.000	0.800 ± 0.0005
	GS	0.005 ± 0.002	0.002 ± 0.000	2.000 ± 0.007
	DS	0.002 ± 0.0005	0.001 ± 0.000	1.000 ± 0.001

GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.