Table 5.
Comparison of Methanomicrobiales percentage obtained by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing
| |
Methanomicrobiales (% ± SD) |
||
|---|---|---|---|
| Sample | qPCR | Shotgun | |
| 50% roughage |
GL |
0.05 ± 0.0002 |
0.08 ± 0.000 |
| DL |
0.38 ± 0.005 |
0.03 ± 0.000 |
|
| GS |
0.02 ± 0.008 |
0.13 ± 0.0005 |
|
| DS |
0.01 ± 0.0002 |
0.23 ± 0.003 |
|
| 75% roughage |
GL |
0.20 ± 0.001 |
0.15 ± 0.0006 |
| DL |
0.60 ± 0.0006 |
0.10 ± 0.000 |
|
| GS |
0.01 ± 0.000 |
0.09 ± 0.000 |
|
| DS |
0.002 ± 0.000 |
0.09 ± 0.000 |
|
| 100% roughage |
GL |
0.06 ± 0.0005 |
0.07 ± 0.0001 |
| DL |
0.16 ± 0.001 |
0.15 ± 0.0006 |
|
| GS |
0.002 ± 0.000 |
0.09 ± 0.0001 |
|
| DS | 0.04 ± 0.0004 | 0.10 ± 0.000 | |
GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.