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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Struct Biol. 2013 Oct 4;184(2):10.1016/j.jsb.2013.09.020. doi: 10.1016/j.jsb.2013.09.020

Figure 3. High-resolution surface metal shadowing of kinesin motors.

Figure 3

Kinesin motors bound to ADP were incubated with microtubules at sub-stoichiometric levels and shadowed with tantalum/tungsten. Dimeric motor constructs are identified by a yellow arrow pairs in A) Kar3Cik1, B) dimeric Ncd, and C) dimeric Eg5 (a kinesin-5). The kinesin-14 motors appear to bind sparsely along the microtubules and span adjacent protofilaments (red relative to yellow arrows in A and B) while dimeric kinesin-5s appear to bind to adjacent tubulin subunits along the same protofilament (red relative to yellow arrows in C).