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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Struct Biol. 2013 Oct 4;184(2):10.1016/j.jsb.2013.09.020. doi: 10.1016/j.jsb.2013.09.020

Figure 7. 3-D structural comparison of Kar3Cik1 and Kar3Vik1.

Figure 7

The 3-D electron density map of Kar3Cik1 was docked into the isosurface mesh representation of the 3-D electron density map of Kar3Vik1. Longitudinal, A) and cross-sectional B) views of the nucleotide-free state show that Kar3Cik1 docks into the Kar3Vik1 map extremely well demonstrating that Kar3Cik1 adopts the same pre-powerstroke configuration as Kar3Vik1. Similarly, longitudinal C) and cross-sectional D) views of the AMPPNP state confirm that Kar3Cik1 fits excellently into the Kar3Vik1 structure showing a nearly identical post-powerstroke position following uptake of ATP. Difference mapping (yellow) reveals locations of density differences between Kar3Vik1 and Kar3Cik1 with a significance of >95%. Interestingly, these results show that overall, Kar3Cik1 and Kar3Vik1 appear structurally identical at this resolution, and it seems that they utilize the same mechanism of movement to perform different functions in the cell. Wire mesh: Kar3Vik1; red/green/blue diffuse density: Kar3Cik1; solid yellow density: difference map at >95% significance.