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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Bone. 2013 Oct 10;58:10.1016/j.bone.2013.10.001. doi: 10.1016/j.bone.2013.10.001

Figure 3. Collagen up-regulates lysyl oxidase via Discoidin Domain Receptor 2 (DDR2) in non-differentiated primary mouse osteoblasts.

Figure 3

Primary mouse osteoblast cultures were transduced with DDR2 shRNA lentivirus and a control shRNA lentivirus. Cells were serum-depleted and then treated with control collagen (0.2 mg/ml) for 24 hours and cell layer proteins were subjected to Western Blotting for (A) DDR2 and (B) 50 kDa prolysyl oxidase and mature 32 kDa lysyl oxidase. Data are means +/− SD, (*, p<0.05, N.S., not significant; ANOVA with post-hoc Tukey; n = 3) from one of two representative experiments with the same outcomes.