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. 2013 Dec 4;8(12):e80861. doi: 10.1371/journal.pone.0080861

Figure 3. Expression of endogenous and exogenous cyclins as a function of Dox and cyclin B.

Figure 3

A: p006-14, p015-4, p016-4 cells and HeLa Tet-Off control cells were transfected with cyclin B1 or control siRNA in the absence or presence of Dox for 18 hr. Cell lysates were immunoblotted with anti- cyclin B1, Cdk1, and β-tubulin antibodies to detect endogenous cyclin B1 and ectopic cyclin B1-EGFP. B: p010-5, p017-4, p023-1 and HeLa Tet-Off control cells were treated as above, except siRNA and antibody were specific for cyclin B2.