Figure 6. Over-expression of B cyclins does not alter G2 and M phase transition times.

P006-14, p015-4 and p010-1, p017-4 cells were cultured with BrdU in the presence (Control) or absence of Dox (Over-express) to test the ability of B cyclin over-expression to decrease G2 transit and affect M transit. Cells were fixed, stained, and measured as in Figure 5. A, C: fractions of unlabeled G2 (circles) or M (squares) cells as a function of time in the absence (white symbols) or presence of Dox (black symbols). B, D: two experiments with p006-14, p015-4, p010-1, and p017-4 were analyzed as in A, C and the transit times calculated. To normalize the data the differences between Dox treated and untreated G2 and G2+M t_1/2 values (dotted lines) were divided by the t_1/2 for Control cells (Y axis). The data for G2 (Delta G2/G) and G2+M (Delta G2+M/G2+M) are denoted on the X axis. The results are not statistically different from 0 for any data set.