Effects of aging, heat stress, and iron manipulation on DMT1 protein expression in the liver. A: Time course evaluation of DMT1 expression after heat stress in young (6 mo) and old (24 mo) rats. * Significant (p<0.05) effect of age; n= 7-9 young and old rats in the nonheated condition and at each time point after heat stress. B: DMT1 expression in saline-treated and iron-treated young rats; n=5-6 rats in the saline and iron-loaded condition. YC: young, saline-treated, nonheated; FeC: young nonheated, iron-loaded. C: Effects of DFO treatment on DMT1 expression in old, nonheated rats; n= 5-6 old rats in the saline-treated and DFO-treated groups, # significant effect of DFO treatment in old rats (p<0.05). OC: old saline-treated, nonheated; DFOC: old nonheated, deferoxamine-treated. Results are expressed as means + SEM.