Skip to main content
. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 25;0:265–272. doi: 10.1016/j.freeradbiomed.2013.04.026

Fig. 5.

Fig. 5

Effect of increasing HSA-to-heme ratios on the protein-radical formation in human plasma by heme/H2O2. (A) Coomassie blue-stained gel to show equal loading, (B) anti-DMPO blotting, and (C) anti-DMPO ELISA. Reactions included 30 μM H2O2, 100 mM DMPO, 30 μM heme, and different concentrations of human plasma, expressed here as HSA in plasma (1.5, 7.5, 30, 60, 90 μM). Reaction mixtures were incubated for 2 h at 37 °C in Chelex-treated, 100 mM phosphate buffer, pH 7.4, containing 25 μM DTPA. Data show representative gel/blot or mean values ±SE from at least three experiments in duplicate (n=3).