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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 25;0:265–272. doi: 10.1016/j.freeradbiomed.2013.04.026

Fig. 7.

Fig. 7

Effect of physiological concentrations of antioxidants (ascorbate, GSH, uric acid, and bilirubin) on the formation of HSA radical by heme/H2O2. (A) Anti-DMPO Western blot and (B) anti-DMPO ELISA. Reactions contained 7.5 μM HSA, 100 mM DMPO, 30 μM H2O2, and 30 μM heme. Samples were incubated for 2 h at 37 °C in Chelex-treated, 100 mM phosphate buffer, pH 7.4, containing 25 μM DTPA. Data show representative blots or mean values ±SE from at least three experiments in duplicate (n=3), and asterisks indicate statistically significant differences (***P<0.001) with respect to samples marked as HSA+Heme+H2O2.