FIGURE 4.

Identification of Bicaudal-C-regulated mRNAs. (A) Reporter mRNAs created with the 3′ UTRs of 14 different Bic-C-associated mRNAs identified by RIP-seq were assayed as described in Figure 1A. Reporter mRNAs containing the xCR1 TCE and the cyclin B1 3′ UTR serve as positive and negative controls, respectively. (B,C) Diagram of vegetal cell-specific repression assay (Zhang et al. 2009). Reporter mRNAs are injected into animal cells or vegetal cells of separate eight-cell embryos. When embryos reach stage 7, luciferase activity is assayed and the ratio in vegetal cells vs. animal cells calculated.