Table 3.
Primers used in genotyping for rotavirus
| Primers | G/P typing | Sequence(5’-3’) | Product (bp) |
|---|---|---|---|
| 9Con1 |
G(+) |
TAGCTCCTTTTAATGTATGG |
- |
| 9Con2 |
G(-) |
GTATAAAATACTTGCCACCA |
905 |
| 9 T1 |
G1(-) |
TCTTGTCAAAGCAAATAATG |
159 |
| 9 T2 |
G2(-) |
GTTAGAAATGATTCTCCACT |
245 |
| 9 T3 |
G3(-) |
GTCCAGTTGCAGTGTTAGC |
467 |
| 9 T4 |
G4(-) |
GGGTCGATGGAAAATTCT |
404 |
| 9T9B |
G9(-) |
TATAAAGTCCATTGCAC |
111 |
| 4Con3 |
P(+) |
TGGCTTCGCCATTTTATAGACA |
- |
| 4Con2 |
P(-) |
ATTTCGGACCATTTATAACC |
877 |
| 1 T1 |
P[8](-) |
TCTACTTGGATAACGTGC |
346 |
| 2 T1 |
P[4](-) |
CTATTGTTAGAGGTTAGAGTC |
484 |
| 3 T1 | P[6](-) | TGTTGATTAGTTGGATTCAA | 268 |