Table 7. Primers used in this study.
| Name | Sequence | Reference | Notes | 5′ coordinatea |
| ef33_734_Rout_2 | CGTAGACCATAACGCAGTATGG | This study | Contig joining | 702,249 |
| ef33_738_Lout_2 | CGCAAGCACTAAACGGCAC | This study | Contig joining | 705,184 |
| ef33_1327_Rout | CGTCTATTAAAGGCTGCATCGG | This study | Contig joining | 1,262,269 |
| ef33_1329_Lout | GGTTCGCATGAGCTTGTATCG | This study | Contig joining | 1,265,356 |
| ef33_829_Rout | TCCACCACTGACATTAGTAGATAC | This study | Contig joining | 798,954 |
| ef33_832_Lout | CAGTACAGCGCGAGATACAATG | This study | Contig joining | 802,162 |
| ef33_124_Lout | ACACCTTCAGAACTTACAGCAATG | This study | Contig joining | 123,860 |
| Thal16SFrev | AGGGTGTGGTTATTGGAGAC | This study | Contig joining | 117,880 |
| Thal_16S-F | GTCTCCAATAACCACACCCT | Kwan et al. | Contig joining | 117,899 |
| 2012 [13] | ||||
| Thal_16S-R | GCTTTCGATACTGCATAGCTC | Kwan et al. | Contig joining | 117,512 |
| 2012 [13] | ||||
| Thal_23S_R | CCTCACGGTACTAGTTCAC | Kwan et al. | Contig joining | 119,618 |
| 2012 [13] | ||||
| Supercontig_9- | GAATAATTGATACTCCAGCTAGC | Kwan et al. | Contig joinint | 114,009 |
| R-out | 2012 [13] | |||
| 27F | AGAGTTTGATCCTGGCTCAG | Weisburg et al. | 16S | 116,943 |
| 1991 [80] | amplification | |||
| 1492R | GGTTACCTTGTTACGACTT | Reysenbach et al. | 16S | 118,390 |
| 1992 [81] | amplification | |||
| M13_4_TOPO-F | GTAAAACGACGGCCAG | Supplied with | TOPO clone | |
| TOPO-TA kit | sequencing | |||
| M13_4_TOPO-R | CAGGAAACAGCTATGAC | Supplied with | TOPO clone | |
| TOPO-TA kit | sequencing | |||
| ef33_829_insert_F | TTGTTAAGTGCATCATGCACCG | This study | Sequencing | 800,604 |
| ef33_832_insert_R | AACAGCAAAACTCAGATAATG | This study | Sequencing | 801,342 |
| ef33_1327_insert_F | ATGAGATAACTGGATACACGC | This study | Sequencing | 1,263,989 |
| ef33_1329_insert_R | TTAGTGTAGGAGTAGTATTGTTC | This study | Sequencing | 1,264,424 |
| ef33_734_insert_F | TCAATCAACAAGAGAGGAGTAC | This study | Sequencing | 703,427 |
| ef33_738_insert_R | AAGACGTCATTTGGCATAGTTAGG | This study | Sequencing | 704,265 |
| ef33_23Sreg_124F2 | ATTCAAATAGCAACCGGGATAAC | This study | Sequencing | 123,190 |
| ef33_23Sreg_124F3 | TAAGGATAATCTGCCATACATAAC | This study | Sequencing | 122,527 |
| ef33_23Sreg_124R1 | CCTGACTGCGAGGCTGAC | This study | Sequencing | 121,371 |
| ef33_23Sreg_C2F2 | CTACCCCCTACTCTGTGCC | This study | Sequencing | 120,807 |
| ef33_23Sreg_R1 | GAGGTCAGCTTACAAGAAAACCG | This study | Sequencing | 118,477 |
a
The coordinates shown refer to the chromosome of Ca. E. faulkneri L5.