Table 1.
Primer sequences for pyrosequencing of six potential methylation biomarkers.
| Genes | Primer sequences for PCR (Forward/Reverse) | Sequencing primers | |
|---|---|---|---|
| HIST1H3J | F | GTTATAAATTTTGGTAGAAGTTATTGT | ATGGTTAGGAAGAAGTAGATAGT |
| R* | ACCTTAATAACCAACTACTTCC | ||
| POU4F2 | F | GGGGAGAGGGGAGTATAA | ATTAGTTTAGATTGATAGTAGAGG |
| R* | AAAAAAAACTATACCAAATTAAACTCACCC | ||
| SHOX2 | F* | TTGGGGGGGTTGGAGTAGTAAA | AACCCCCTAAATTCTTCCAT |
| R | CTCCTTCTTCTCCTTCACTTTCTAATC | ||
| PHKG2 | F | GTTTGTAATTTTAGTATTTTGGGAGGTTGA | AAGTTTAAGGTTGTAGTGA |
| R* | TCCCTAACTAAATTCAACATTTTCTCTT | ||
| TLX3 | F | TGGTTGAGGTAGGAGAGGAATTAGTA | GGTTTAAGAAAGATGATATAGAG |
| R* | CACTAAAACTTTACCAAAAATAC | ||
| HOXA7 | F* | GGGAGTAAAGGAGTAAGAAGT | CAACAAATCACAAATCAAAATTA |
| R | ACCCAACAACAAATCACAAATCAAAATT | ||
*
Primers with 5 ′-biotin tag.