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. 2013 Dec 5;4:271. doi: 10.3389/fgene.2013.00271

Table 1.

Primer sequences for pyrosequencing of six potential methylation biomarkers.

Genes Primer sequences for PCR (Forward/Reverse) Sequencing primers
HIST1H3J F GTTATAAATTTTGGTAGAAGTTATTGT ATGGTTAGGAAGAAGTAGATAGT
R* ACCTTAATAACCAACTACTTCC
POU4F2 F GGGGAGAGGGGAGTATAA ATTAGTTTAGATTGATAGTAGAGG
R* AAAAAAAACTATACCAAATTAAACTCACCC
SHOX2 F* TTGGGGGGGTTGGAGTAGTAAA AACCCCCTAAATTCTTCCAT
R CTCCTTCTTCTCCTTCACTTTCTAATC
PHKG2 F GTTTGTAATTTTAGTATTTTGGGAGGTTGA AAGTTTAAGGTTGTAGTGA
R* TCCCTAACTAAATTCAACATTTTCTCTT
TLX3 F TGGTTGAGGTAGGAGAGGAATTAGTA GGTTTAAGAAAGATGATATAGAG
R* CACTAAAACTTTACCAAAAATAC
HOXA7 F* GGGAGTAAAGGAGTAAGAAGT CAACAAATCACAAATCAAAATTA
R ACCCAACAACAAATCACAAATCAAAATT
*

Primers with 5 ′-biotin tag.