Table 2.
Primer sequences used for mutation analysis (MALDI-TOF-MS assays).
| Mutation sites | Primer sequences (Forward/Reverse) | Extend primers | |
|---|---|---|---|
| BRAF_1799 | F | ACGTTGGATGTTCAAACTGATGGGACCCAC | TGATTTTGGTCTAGCTACAG |
| R | ACGTTGGATGTCTTCATGAAGACCTCACAG | ||
| HRAS_34 | F | ACGTTGGATGAATGGTTCTGGATCAGCTGG | ACTCTTGCCCACACCGC |
| R | ACGTTGGATGGACGGAATATAAGCTGGTGG | ||
| HRAS_35 | F | ACGTTGGATGAATGGTTCTGGATCAGCTGG | AGCGGGTGGTGGTGGGCGCCG |
| R | ACGTTGGATGGACGGAATATAAGCTGGTGG | ||
| HRAS_37 | F | ACGTTGGATGAATGGTTCTGGATCAGCTGG | TCATCGCACTCTTGCCCACAC |
| R | ACGTTGGATGGACGGAATATAAGCTGGTGG | ||
| HRAS_38 | F | ACGTTGGATGAATGGTTCTGGATCAGCTGG | CAGCGCACTCTTGCCCACA |
| R | ACGTTGGATGGACGGAATATAAGCTGGTGG | ||
| HRAS_181 | F | ACGTTGGATGTGGCAAACACACACAGGAAG | CATGGCGCTGTACTCCTCCT |
| R | ACGTTGGATGTGTTGGACATCCTGGATACC | ||
| HRAS_182 | F | ACGTTGGATGTGGCAAACACACACAGGAAG | CATGGCGCTGTACTCCTCC |
| R | ACGTTGGATGTGTTGGACATCCTGGATACC | ||
| HRAS_183 | F | ACGTTGGATGTGGCAAACACACACAGGAAG | CGCATGGCGCTGTACTCCTC |
| R | ACGTTGGATGTGTTGGACATCCTGGATACC | ||
| KRAS_34 | F | ACGTTGGATGTAGCTGTATCGTCAAGGCAC | ACTCTTGCCTACGCCAC |
| R | ACGTTGGATGAGGCCTGCTGAAAATGACTG | ||
| KRAS_35 | F | ACGTTGGATGTAGCTGTATCGTCAAGGCAC | CTGTGGTAGTTGGAGCTG |
| R | ACGTTGGATGAGGCCTGCTGAAAATGACTG | ||
| KRAS_37 | F | ACGTTGGATGTAGCTGTATCGTCAAGGCAC | GAGGGGCACTCTTGCCTACGC |
| R | ACGTTGGATGAGGCCTGCTGAAAATGACTG | ||
| KRAS_38 | F | ACGTTGGATGTAGCTGTATCGTCAAGGCAC | AGGCACTCTTGCCTACG |
| R | ACGTTGGATGAGGCCTGCTGAAAATGACTG | ||
| NRAS_181 | F | ACGTTGGATGTCGCCTGTCCTCATGTATTG | ATACTGGATACAGCTGGA |
| R | ACGTTGGATGCCTGTTTGTTGGACATACTG | ||
| NRAS_182 | F | ACGTTGGATGTCGCCTGTCCTCATGTATTG | ATGGCACTGTACTCTTCT |
| R | ACGTTGGATGCCTGTTTGTTGGACATACTG | ||
| NRAS_183 | F | ACGTTGGATGTCGCCTGTCCTCATGTATTG | CTGGATACAGCTGGACA |
| R | ACGTTGGATGCCTGTTTGTTGGACATACTG | ||