Table 3.
Primer sequences for real-time RT-PCR in gene re-expression analysis.
| Gene | Primer sequences (Forward/Reverse) | Anneal (°C) | Product (bp) | |
|---|---|---|---|---|
| HIST1H3J | F | AAATCAAGCAGAGGCGAAGTCGGA | 58 | 106 |
| R | GGATAGTGGGTCTCGTCAAAAAGC | |||
| POU4F2 | F | CACCAAGCCTGAACTCTTCAAT | 58 | 101 |
| R | GCTGAATGGCAAAGTAGGCTTCG | |||
| SHOX2 | F | AAATCAAGCAGAGGCGAAGTCGGA | 58 | 85 |
| R | GGATAGTGGGTCTCGTCAAAAAGC | |||
| PHKG2 | F | TGATCTTGTTCACACTCCTGGCT | 58 | 145 |
| R | GAGATCAGGTCTTTGACAGTGCT | |||
| TLX3 | F | CTGTCTGCACAACTCGTCACTCTT | 60 | 79 |
| R | GACAGCGGGAACCTTGGAACTATC | |||
| HOXA7 | F | AGTTCCACTTCAACCGCTACCTGAC | 58 | 131 |
| R | GTCCTTATGCTCTTTCTTCCACTTC | |||