Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 10;14:219. doi: 10.1186/1471-2369-14-219

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Brandenburg et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Histological and immunohistochemical analyis of calcified and non-calcified valves from dialysis patients. Von Kossa staining was strongly positive in calcified valves from dialysis patients (Ai) and completely negative in all non-calcified control valves (Aii). Immunhistochemical staining for sclerostin revealed a strong expression in the calcified areas (arrows in Bi, calcification marked with asterisk) and a faint sclerostin expression in non-calcified areas (Ci, arrows) of the calcified valves from dialysis patients. The immunohistochemistry for sclerostin was negative in all non-calcified valves (Bii, Cii). Qt-RT PCR for sclerostin expression showed a highly significant upregulation of sclerostin expression in the calcified valves compared to the non-calcified valves (D). Gene expression in the non-calcified control valves was set as 1. All scale bars 50 ηm.