Table 1. The information of each primary antibody used in the study.
| Antibodies | Source | Dilutions | Manufacturer | Catalog# | Application |
|---|---|---|---|---|---|
| Akt1 | Rabbit | 1:1,000 | Cell Signaling | 2938 | WB |
| Akt2 | Rabbit | 1:1,000 | Cell Signaling | 3063 | WB |
| Akt3 | Rabbit | 1:500 | Cell Signaling | 3788 | WB |
| pAkt (Ser473) | Rabbit | 1:100/1:1,000 | Cell Signaling | 9271 | IF/WB |
| Akt | Rabbit | 1:1,000 | Cell Signaling | 9272 | WB |
| pFKHR (Ser256) | Rabbit | 1:1,000 | Cell Signaling | 9461 | WB |
| FKHR | Rabbit | 1:50/1:1,000 | Cell Signaling | 2880 | IF/WB |
| pPRAS40 (Thr246) | Rabbit | 1:100/1:1,000 | Cell Signaling | 2997 | IF/WB |
| PRAS40 | Rabbit | 1:1,000 | Cell Signaling | 2691 | WB |
| pmTOR (Ser2448) | Rabbit | 1:1,000 | Cell Signaling | 2971 | WB |
| mTOR | Rabbit | 1:1,000 | Cell Signaling | 2983 | WB |
| GFP | Rabbit | 1:500 | Cell Signaling | 2956 | WB |
| pPTEN (Ser380) | Rabbit | 1:1,000 | Cell Signaling | 9551 | WB |
| PTEN | Rabbit | 1:1,000 | Cell Signaling | 9552 | WB |
| pGSK3β (Ser 9) | Rabbit | 1:1,000 | Cell Signaling | 9336 | WB |
| GSK3β | Rabbit | 1:1,000 | Cell Signaling | 9332 | WB |
| pS6K p70 (Ser371) | Rabbit | 1:500 | Cell Signaling | 9208 | WB |
| HA | Rabbit | 1:100 | Cell Signaling | 3724 | IF |
| MAP-2 | Mouse | 1:200 | Sigma | M-4403 | IF |
| β-Actin | Mouse | 1:300 | Sigma | A-5441 | WB |
IF, immunofluorescence; WB, western blots.