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. 2013 Sep 4;33(12):1976–1982. doi: 10.1038/jcbfm.2013.157

Figure 5.

Figure 5

Pretreatment with miR-181a antagomir increases CA1 neuronal survival after forebrain ischemia. (A) miR-181a levels 1 and 3 days after injecting miR-181a antagomir or mismatch miR-181a antagomir just outside the hippocampus relative to sham. n=5 to 6. *P<0.05 and **P<0.01 compared with sham. (B) Representative blots of Bcl-2 protein expression after transfection with indicated antagomir in vivo. (C) Levels of Bcl-2 protein 1 and 3 days after injecting miR-181a antagomir or mismatch miR-181a antagomir relative to sham. n=3 to 6. *P<0.05 compared with sham. (D) Cresyl violet staining of hippocampi. Sham, which was not subjected to ischemia, shows a normal hippocampus. After forebrain ischemia in a rat pretreated with mismatch miR-181a antagomir, selective neuronal loss of CA1 hippocampal neurons (between white arrows) and reduced neuronal loss (between white arrows) in a rat treated with miR-181a antagomir outside the left hippocampus is shown. (E) Quantitation of neuronal survival in CA1 of rats treated with miR-181a antagomir (Ant) or mismatch miR-181a antagomir (Mis) relative to sham. n=15. ***P <0.001, compared with rats treated with mismatch miR-181a antagomir.