Table 1.
Isolation and identification of AIV type A from cloacal swabs during the period of December’2009 to February’2012
| Winter season | Division | Wintering sites | Total no. of sample | No. of HA positive samples | No. of NDV positive samples | No. of AIV positive cases in NDV positive samples | RT-PCR positive samples of AIV | Prevalence of AIV (%) | Seasonal prevalence (%) |
|---|---|---|---|---|---|---|---|---|---|
| |
Sylhet |
Hakaluki Haor |
450 |
278 |
126 |
19 (15.05)* |
146 |
32.44 |
26.89 |
| Dec. 2009 to Feb. 2010 |
|
Tanguar Haor |
225 |
94 |
52 |
6 (11.54) |
51 |
22.67 |
|
| |
Dhaka |
Jahangirnagar Lake |
225 |
73 |
37 |
4 (10.81) |
45 |
20.00 |
|
| |
Sylhet |
Hakaluki Haor |
300 |
145 |
75 |
7 (9.33) |
63 |
21.00 |
18.50 |
| Dec. 2010 to Feb. 2011 |
|
Tanguar Haor |
150 |
64 |
39 |
3 (7.69) |
27 |
18.00 |
|
| |
Dhaka |
Jahangirnagar Lake |
150 |
58 |
23 |
2 (8.69) |
21 |
14.00 |
|
| |
Sylhet |
Hakaluki Haor |
300 |
163 |
75 |
5 (6.67) |
58 |
19.33 |
18.33 |
| Dec. 2011 to Feb. 2012 |
|
Tanguar Haor |
150 |
71 |
37 |
2 (5.41) |
23 |
15.33 |
|
| |
Dhaka |
Jahangirnagar Lake |
150 |
73 |
41 |
2 (4.88) |
29 |
19.33 |
|
| Total | 2100 | 1019 | 505 | 50 (9.90%) | 463 | 22.05 |
A total of 2100 cloacal swab samples from semi-scavenging domestic ducks were tested for virus isolation through chicken embryo inoculation following by Haemagglutination test and identification was confirmed by RT-PCR from HA positive samples (allantoic fluid).
*Values in the parenthesis indicates per cent positive.