Table 1.

Escherichia coli strains and plasmids utilized in this report

Strains
Name	Characteristics	Reference
JM101	F´ traD36 proA + proB + lacIq lacZΔM15/supE thi Δ(lac-proAB) rpoS(33 am)	Messing [41]
PB11	JM101 Δ(ptsH, ptsI, crr)::kan	Flores et al. [21]; Flores et al. [22]
PB12	PB11, PTS- Glc+; laboratory-evolved strain	Flores et al. [21]; Flores et al. [22]
AR2	PB12 lacI-aroK-aroL-	This work
AR3	PB12 lacI-aroK-aroL-pykF-	This work
AR26	AR2 + pTrcAro6 (Trc/aroB+tktA+aroGfbr+aroE+aroD+zwf+)	This work
AR36	AR3 + pTrcAro6 (Trc/aroB+tktA+aroGfbr+aroE+aroD+zwf+)	This work
AR2e	AR2 + pTrc327par (plasmid vector without synthetic operon)	This work
AR3e	AR3 + pTrc327par (plasmid vector without synthetic operon)	This work
Plasmids
Name	Characteristics	Reference
pKD3	PCR template for amplification of chloramphenicol resistance gene flanked by homologous sequences	Datsenko and Wanner [42]
pKD46	Plasmid expressing λ-Red recombinase system with thermosensitive origin of replication	Datsenko and Wanner [42]
pCP20	FLP recombinase expression plasmid	Cherepanov and Wackernagel [43]
pBR327par	Derivative of pBR322 exhibiting increased copy number and segregational stability	Zurita et al. [32]
pTrc99A	Multipurpose expression plasmid bearing lacI gene and a polylinker in front of Trc promoter	Amann et al. [31]
pTrc327par	Contains the promoter, polylinker, and terminators of pTrc99A, and par and ori regions of pBR327par	This work (Figure 2)
pTrcAro6	pTrc327par containing a 6-gene synthetic operon to enhance the production of shikimate	This work (Figure 2)