Table 3.

Comparison of measured metabolites and calculated kinetic and stoichiometric parameters between three fermentations of strain AR36 with different substrate concentrations

Strain	AR36	AR36	AR36
Culture conditions	Batch 50 g/L Glc + 15 g/L YE	Batch 100 g/L Glc + 15 g/L YE	Batch 100 g/L Glc + 30 g/L YE
SA titer (g/L)	23.80 ± 0.00	41.80 ± 2.83	43.30 ± 0.57
Glc consumed (g/L)	52.65 ± 1.20	103.70 ± 6.79	105.55 ± 4.45
Duration of culture (h)	32	60	30
YSA/Glc (mol/mol)	0.47 ± 0.01	0.42 ± 0.00	0.42 ± 0.01
Acetate titer (g/L)	1.45 ± 0.00	11.90 ± 0.14	8.65 ± 0.92
Xmax (g/L)	6.30 ± 0.09	6.54 ± 0.09	12.54 ± 0.06
μ (h-1)	0.53 ± 0.03	0.45 ± 0.02	0.45 ± 0.00
Qpglobal (gSA/L*h)	0.74 ± 0.00	0.75 ± 0.07	1.44 ± 0.02
Qsglobal (gGlc/L*h)	-1.65 ± 0.04	-1.74 ± 0.11	-3.52 ± 0.15
qpexp (gSA/gDCW*h)	0.38 ± 0.00	0.34 ± 0.03	0.46 ± 0.06
qsexp (gGlc/gDCW*h)	-1.14 ± 0.15	-1.11 ± 0.00	-1.18 ± 0.03
qpsta (gSA/gDCW*h)	0.16 ± 0.01	0.19 ± 0.02	0.20 ± 0.01
qssta (gGlc/gDCW*h)	-0.36 ± 0.01	-0.42 ± 0.01	-0.42 ± 0.03

Y_SA/Glc = SA yield from Glc; X_max = maximum attained biomass expressed as dry cell weight; Qp_global = volumetric SA productivity for the entire fermentation; Qs_global = volumetric Glc consumption rate for the entire fermentation; qp_exp = specific SA productivity for exponential phase; qs_exp = specific Glc consumption rate for exponential phase; qp_sta = specific SA productivity for stationary phase; qs_sta = specific Glc consumption rate for stationary phase; μ = specific growth rate.