Table 1. Compounds structurally identified from earthworm, Metaphire.posthuma by GC-MS.
| Rt (min) | compound | fragmentations (m/z) | Idn | Rt (min) | compound | fragmentations (m/z) | Idn |
| Amino acids & their derivatives | Fatty acids | ||||||
| 9.16 | L-alanine | 116,73,147,190,59 | A | 21.41 | Tridecanoic acid | 117,73,271,129,145 | A |
| 9.48 | L-glycine | 102,73,147,75,204 | A | 21.54 | Tetradecanoic acid | 285,117,75,129,145 | A |
| 11.53 | L-valine | 144,73,218,145,100 | A | 19.03 | Tri decanoic acid methyl ester | 74,87,55,143,185 | A |
| 12.23 | L-leucine | 158,73,147,102,59 | A | 19.58 | Dodecanoicacid | 75,117,257,132,145 | A |
| 12.34 | L-isoleucine | 158,73,218,147,232 | A | 26.98 | Hexadecanoic acid | 117,313,73,129,145 | A |
| 14.70 | L-serine | 204,73,218,147,100 | A | 27.71 | 9,12-octa deca dienoic acid (Z,Z) methyl ester | 67,81,95,55,109 | B |
| 15.29 | L-threonine | 73,117,218,147,101 | A | 27.80 | 9-Octadecenoic acid (Z)methyl ester | 55,74,69,83,97 | B |
| 17.90 | L-methionine | 176,128,73,61,147 | A | 27.90 | 11-octadecenoic acid (E) methyl ester | 55,69,74,83,97 | B |
| 17.75 | L-aspartic acid | 73,232,100,147,218 | A | 28.24 | Octadecanoic acid methyl ester | 74,87,143,298,255 | B |
| 19.87 | L-glutamic acid | 246,73,128,147,156 | A | 28.46 | Heptadecanoic acid | 73,117,327,132,145 | A |
| 17.82 | L-lysine | 84,73,156,102,128 | A | 28.98 | 6-Hexadecenoic acid-7-methyl ester (Z) | 138,55,69,97,83 | B |
| 18.47 | L-phenylalanine | 218,192,73,91,147 | A | 29.45 | 9,12-octa deca dienoic acid (Z,Z) | 75,81,67,129,337 | B |
| 22.23 | L-ornithine | 174,73,142,186,348 | A | 29.61 | 11-Cis octadecenoic acid | 339,73,117,129,55 | B |
| 24.43 | L-tyrosine | 218,73,280,100,147 | A | 29.94 | Octadecanoic acid | 117,341,73,132,145 | B |
| 23.94 | N-α-acetyl-L- lysine | 174,73,156,86,59 | B | 30.16 | 8,11,14-Eicosa trienoic acid mehtyl ester (Z,Z,Z) | 79,67,93,55,150 | A |
| 18.09 | pyroglutamic acid | 156,73,147,230,258 | B | 30.24 | 5,8,11,14-Eicosa tetraenoic acid methyl ester(all Z) | 79,91,67,105,55 | B |
| Organic acids | 30.37 | 5,8,11,14,17-Eicosa pentaenoic acid methyl ester(all Z) | 79,91,67,105,119 | B | |||
| 8.09 | pyruvic acid | 73,174,45,89,59 | A | 30.62 | Cis-13-Eicosenoic acid methyl ester | 55,69,74,83,97,297 | A |
| 13.59 | scuccinic acid | 147,73,247,129,45 | A | 31.47 | Arachidonic acid | 73,91,67,117,55 | A |
| 14.29 | 2-butenedioic acid | 245,147,73,45,83 | B | 31.55 | Cis-5,8,11,14,17-Eicosapenta enoic acid | 79,73,91,117,67 | B |
| 16.43 | malonic acid | 73,147,305,45,69 | A | 31.70 | α-Linoleic acid | 73,79,67,95,55 | A |
| 17.44 | malic acid | 73,147,233,245,133 | A | 32.45 | Cis-7,10,13,16-Docosa tetra enoic acid methyl ester | 79,91,67,105,55 | B |
| 18.07 | 2-ketoisovaleric acid | 73,147,157,232,260 | B | 33.45 | 2-mono palmitin | 129,218,73,147,103 | B |
| 19.08 | glutaric acid | 73,147,198,156,288 | B | 33.76 | Eicosanoic acid glycerate ester | 73,57,147,43,129 | B |
| 11.01 | 2-butenoic acid | 147,73,231,45,66 | B | 33.67 | Hexadecanoic acid glycerate ester | 371,460,239,73,147 | B |
| polyols (poly hydric alcohols) | |||||||
| 8.26 | lactic acid | 147,117,73,191,133 | A | 12.78 | glycerol | 73,147,205,117,103 | A |
| 19.05 | 2-hydroxy glutaric acid | 73,129,147,247,349 | B | 27.19 | Inositol | 73,318,147,217,305 | A |
| 26.72 | d-(+)gluconicacid | 73,319,147,129,220 | B | 28.15 | myo Inositol | 73,217,147,305,191 | A |
| Carbohydrates | 21.22 | threitol | 73,147,103,205,217 | B | |||
| 24.31 | fructose | 73,103,217,307,147 | A | 25.65 | mannitol | 73,319,147,205,217 | A |
| 25.30 | glucose | 73,319,205,147,218 | A | 31.38 | 1-O-pentadecylglycerol | 205,147,73,117,131 | B |
| 25.16 | mannose | 73,147,319,205,160 | A | 32.78 | 1-O-hexadecylglycerol | 205,147,117,73,133 | B |
| 25.47 | Galactose | 73,205,319,147,217 | A | Phosphates | |||
| 34.22 | turanose | 73,361,147,217,103 | B | 12.98 | phosphoric acid | 299,73,133,314,211 | B |
| 35.02 | lactose | 204,73,191,217,361 | A | 10.78 | methyl phosphate | 241,73,133,256,211 | B |
| 35.51 | maltose | 204,73,191,361,217 | A | 22.80 | 1-glycero phosphate | 357,73,299,147,103 | B |
| 34.79 | sucrose | 361,73,217,147,271 | A | 22.96 | O-phospho ethanolamine | 73,299,188,174,315 | B |
| 35.69 | melibiose | 204,73,191,217,129 | B | galactose-6-phosphate | 73,299,387,315,147 | B | |
| 26.47 | talose | 204,73,191,217,147 | B | amines | |||
| 24.85 | methyl mannopyranoside | B | 21.95 | cadaverine | 174,73,86,59,100 | B | |
| Others | 9.59 | ethanolamine | 174,73,147,100,86 | B | |||
| 39.31 | cholesterol | 129,329,368,73,353 | A | 34.57 | N-acetyl glucosamine | 73,147,103,205,129 | B |
| 36.50 | cholesta-3,5-diene | 368,147,105,91,145 | B | 14.67 | 2-amino-3-phenyl propane | 188,73,100,147,114 | B |
| 25.91 | Indole acetic acid | 202,319,73,188,154 | B |
Rt = retention time, Idn = identification, A = These compounds are confirmed by authentic standards, B = These compounds are identified by NIST library Mass Spectral fragmentation pattern.