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. 2013 Dec 4;8(12):e81158. doi: 10.1371/journal.pone.0081158

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© 2013 Hägglund et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunohistochemical analysis for the presence of BrdU-labelled cells in the prospective neural retina on a coronal section of an E10.5 control embryo (left panel) and an Lhx2-Cre:β-catenin^flox/flox embryo (right panel). The part of the prospective neural retina that was analysed for BrdU⁺ cells in the control and mutant embryos are outlined. (B) Immunohistochemical analysis for the presence apoptotic cells as revealed by the presence of activated Casp-3⁺ cells on coronal section of an E10.5 control embryo (left panel) and a Lhx2-Cre:β-catenin^flox/flox embryo (right panel). (C) Immunohistochemical analyses for the presence of phosphorylated myosin light chain 2 (pMLC2) on a coronal section of an E10.5 control embryo. (D) Immunohistochemical analyses for the presence of pMLC2 on a coronal section on an E10.5 Lhx2-Cre:β-catenin^flox/flox embryo. Insets in C and D are a magnification of the areas indicated by the squares. Scale bars: (A, B and C, D) 100 µm.