Figure 8.

Dependence of average puff amplitude and the largest puff amplitude on the number of IP₃R at a puff site. Amplitudes are normalized to mean blip amplitude, 1.6. A linear fit is performed for $N_{\text{IPR}}=3\sim 11$, whereas a square root function gives an excellent fit to the points of $N_{\text{IPR}}\ge 12$. The average puff amplitude is expressed as mean ± SD. We set $p=0.1$μM and $a_{h_{42}}=1$ s⁻¹.