Table 1.
Effect of selected medicinal plants on apoptosis induced by STZ in RINm5F cells
| Groups under study | % Cells in Pre G1 (M1) | % Cells in G0, G1 (M2) | % Cells in G2,M,S (M3) |
|---|---|---|---|
| Cell control |
8.18 ± 1.41 |
52.46 ±6.99 |
26.419 ±2.56 |
| Cells + Pe (2.5 μg/ml) |
11.81 ±2.98 |
52.03 ±17.41 |
27.51 ± 9.19 |
| Cells + Pe (5 μg/ml) |
11.51 ±2.98 |
51.25 ±3.22 |
27.75 ±3.53 |
| Cells + Pe (10 μg/ml) |
16.03 ±4.52* |
48.01 ±8.50 |
25.60 ±4.66 |
| Cells + Pe (25 μg/ml) |
17.39 ±1.82 |
49.05 ±5.53 |
25.97 ±1.96 |
| Cells + Pe (50 μg/ml) |
18.33 ±1.62* |
45.79 ±3.17 |
28.47 ±1.99 |
| Cells + Pe (100 μg/ml) |
20.63 ±1.60*** |
44.01 ±2.35 |
26.29 ±1.58 |
| Cells + STZ |
25.48 ± 3.77*** |
39.13 ±6.69* |
36.18 ±8.48 |
| Cells + Pe (2.5 μg/ml) + STZ |
19.25 ±3.45 |
41.25 ±6.69 |
31.02 ±8.75 |
| Cells + Pe (5 μg/ml) + STZ |
15.29 ±4.05$$$ |
37.86 ±6.77 |
34.75 ±8.34 |
| Cells + Pe (10 μg/ml) + STZ |
11.77 ±2.31$$$ |
45.28 ±5.61 |
29.13 ±7.98 |
| Cells + Pe (25 μg/ml) + STZ |
16.04 ±1.42$$$ |
38.65 ±3.43 |
35.40 ±2.99 |
| Cells + Pe (50 μg/ml) + STZ |
18.34 ±1.23$ |
36.89 ±4.33 |
29.86 ±5.26 |
| Cells + Pe (100 μg/ml) + STZ |
23.35 ±1.80 |
36.90 ±4.15 |
30.29 ±4.29 |
| Cell control |
9.58 ± 2.48 |
56.03 ± 2.65 |
25.51 ± 2.05 |
| Cells + Cl (2.5 μg/ml) |
11.05 ± 2.89 |
51.28 ± 3.32 |
26.82 ± 4.27 |
| Cells + Cl (5 μg/ml) |
10.36 ± 3.94 |
51.24 ± 2.88 |
28.41 ± 3.41 |
| Cells + Cl (10 μg/ml) |
10.94 ± 3.78 |
51.75 ± 5.09 |
28.81 ± 4.81 |
| Cells + Cl (25 μg/ml) |
12.98 ± 2.72 |
46.02 ± 7.77 |
30.61 ± 1.71 |
| Cells + Cl (50 μg/ml) |
14.7 ± 2.06 |
48.61 ± 4.63 |
31.94 ± 2.61 |
| Cells + STZ |
23.37 ± 4.51*** |
41.38 ± 7.44 |
32.21 ± 6.86 |
| Cells + Cl (2.5 μg/ml) + STZ |
17.14 ± 4.75$$ |
41.94 ± 5.02 |
31.21 ± 8.91 |
| Cells + Cl (5 μg/ml) + STZ |
15.77 ± 5.13$$$ |
40.82 ± 2.87 |
32.73 ± 7.59 |
| Cells + Cl (10 μg/ml) + STZ |
12.74 ± 3.07$$ |
44.60 ± 3.09 |
29.62 ± 7.73 |
| Cells + Cl(25 μg/ml) + STZ |
18.74 ± 2.44 |
42.42 ± 1.93 |
35.47 ± 1.38 |
| Cells + Cl (50 μg/ml) + STZ |
19.13 ± 3.99 |
40.18 ± 3.48 |
32.40 ± 1.67 |
| Cell control |
5.87 ± 1.70 |
53.22 ± 11.81 |
26.98 ± 7.17 |
| Cells + Tc 2.5 μg/ml |
7 ± 1.57 |
57.79 ± 13.81 |
23.90 ± 6.57 |
| Cells + Tc 5 μg/ml |
5.92 ± 1.91 |
59.32 ± 13.48 |
23.98 ± 7.96 |
| Cells + Tc 10 μg/ml |
7.12 ± 2.22 |
57.12 ± 12.35 |
24.58 ± 7.44 |
| Cells + Tc 25 μg/ml |
7.62 ± 2.17 |
57.13 ± 3.45 |
25.73 ± 6.11 |
| Cells + Tc 50 μg/ml |
7.07 ± 2.44 |
60.44 ± 3.89 |
20.03 ± 5.79 |
| Cells + Tc100 μg/ml |
10.16 ± 5.27 |
56.95 ± 2.47 |
28.68 ± 0.69 |
| Cells + STZ |
24.69 ± 2.19*** |
40.01 ± 9.17* |
26.28 ± 1.67 |
| Cells + Tc2.5 μg/ml + STZ |
26.69 ± 1.34 |
51.35 ± 6.22 |
18.73 ± 8.09 |
| Cells + Tc 5 μg/ml + STZ |
25.86 ± 1.83 |
35.08 ± 10.81 |
34.82 ± 6.61 |
| Cells + Tc 10 μg/ml + STZ |
21.65 ± 3.34 |
43.86 ± 9.22 |
25.21 ± 8.91 |
| Cells + Tc 25 μg/ml + STZ |
16.77 ± 3.65$$ |
43.48 ± 2.13 |
31.36 ± 3.86 |
| Cells + Tc 50 μg/ml + STZ |
14.16 ± 1.69$ |
42.86 ± 6.94 |
28.54 ± 6.12 |
| Cells + Tc100 μg/ml + STZ |
14.05 ± 1.80$$ |
48.64 ± 1.3 |
36.12 ± 1.78 |
| Cell control |
11.87 ± 3.70 |
53.22 ± 11.81 |
26.98 ± 7.17 |
| Cells + Gb (1 μg/ml) |
12.6 ± 2.39 |
49.79 ± 9.11 |
26.87 ± 6.33 |
| Cells + STZ |
28.5 ± 8.85*** |
40.24 ± 6.71* |
28.42 ± 8.30 |
| Cells + STZ + Gb (1 μg/ml) | 19.91 ± 5.05$ | 47.3 ± 5.92 | 25.84 ± 3.12 |
RINm5F cells were treated with different concentrations of plants extracts in presence and absence of STZ. Apoptosis was measured using cell cycle analysis from the DNA histograms generated on the Flow Cytometer followed by PI staining. All values are indicative of Mean ± SD. *p < 0.05;***p < 0.001 as compared to Untreated cells, $p < 0.05; $$p < 0.01; $$$p < 0.001 as compared to STZ treated cells. (ANOVA followed by post hoc test).