Table 1.
Primer pairs used for RT-PCR experiment
| Gene name | Sequence (5′ → 3′) | Genbank accession | |
|---|---|---|---|
| NR1: |
sense |
TATGGAGAAGCACAACTACGAGAG |
NM_000832 |
| |
antisense |
CGAGCAGCAGGACCCATCAGTGT |
|
| NR2A |
sense |
CGGGTATGATTTCTTCTGGATTGTCCC |
NM_000833 |
| |
antisense |
GGGTGACGATGCTGAGATGGTTGT |
|
| NR2B |
sense |
AGCCCCATCATTCTTCTTTACTGTACCAAG |
NM_000834 |
| |
antisense |
CCTTTCCCACTTCCTCTCCTTGTTCAG |
|
| NR2C |
sense |
CTTTGTGGAGACGGGCATCAGTGT |
NM_000835 |
| |
antisense |
GGCGAGGAAGATGACAGCAAAGAA |
|
| NR2D |
sense |
CCTGCTGCGTGATTATGGTTTCCT |
NM_000836 |
| |
antisense |
GAGGGCTGTGGGTTCGGTTGA |
|
| NR3A |
sense |
CTTTTTAGCAGCCTCCATAGCAGTAATGA |
NM_133445 |
| |
antisense |
TCATACAGAGTGAGGAAGACGGCAGTG |
|
| NR3B |
sense |
GTATCAACTCCGCCCGCTCACAG |
NM_138690 |
| |
antisense |
AGAGGATGGCGTAGCACAGGTTGA |
|
| β-actin |
sense |
CTAACTTGCGCAGAAAACAAGAT |
NM_001101 |
| antisense | TTCCTGTAACAACGCATCTCATA |
Sequences of the human NMDA receptor subunits were obtained from GENBANK, and PCR primers were designed.